Anticorps Polyclonal de lapin anti-OXA1L

• Phare
• Validé par KD/KO

OXA1L Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 21055-1-AP

Synonymes

Hsa, OXA1 like protein, OXA1Hs, OXA1L

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Galerie de données de validation

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  WB analysis using 21055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21055-1-AP (OXA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21055-1-AP

  WB result of OXA1L antibody (21055-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXA1L transfected HeLa cells.

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  WB analysis of NIH/3T3 using 21055-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21055-1-AP (OXA1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 21055-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OXA1L antibody (21055-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 21055-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 21055-1-AP (OXA1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules L02, cellules NIH/3T3, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

21055-1-AP cible OXA1L dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OXA1L Protéine recombinante Ag13819
• Nom complet: oxidase (cytochrome c) assembly 1-like
• Masse moléculaire calculée: 437 aa, 49 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC001669
• Symbole du gène: OXA1L
• Identification du gène (NCBI): 5018
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation.; Authors - Prashant Mishra
human	WB,IP	Nat Commun; LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.; Authors - Chun-Shik Shin
human	WB	Am J Hum Genet; Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number.; Authors - Kyle Thompson
human	WB	EMBO Mol Med; OXA1L mutations cause mitochondrial encephalopathy and a combined oxidative phosphorylation defect.; Authors - Kyle Thompson ; KD Validated
mouse	WB	Aging (Albany NY); Reduced mitochondrial translation prevents diet-induced metabolic dysfunction but not inflammation.; Authors - Kara L Perks
mouse	WB	J Cell Sci; Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis; Authors - Richard G. Lee

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

• Applications: Western Blot