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Anticorps Polyclonal de lapin anti-OTUD6B

OTUD6B Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 25430-1-AP

Synonymes

CGI 77, DUBA 5, DUBA5, OTU domain containing 6B, OTUD6B

Galerie de données de validation

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  • Western Blot (WB) analysis of HeLa cells using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis of HeLa using 25430-1-AP

    WB result of OTUD6B antibody (25430-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD6B transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis using 25430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis of MCF-7 using 25430-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis of Jurkat using 25430-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis of HEK-293 using 25430-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using OTUD6B Polyclonal antibody (25430-1-AP)

    IHC staining of human ovary tumor using 25430-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25430-1-AP (OTUD6B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using OTUD6B Polyclonal antibody (25430-1-AP)

    IHC staining of human ovary tumor using 25430-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25430-1-AP (OTUD6B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using OTUD6B Polyclonal antibody (25430-1-AP)

    IF Staining of PC-3 using 25430-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25430-1-AP (OTUD6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Applications testées

Résultats positifs en WBcellules HEK-293, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules PC-3
Résultats positifs en IHCtissu de tumeur ovarienne humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules PC-3

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochimie (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

25430-1-AP cible OTUD6B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène OTUD6B Protéine recombinante Ag22044
Nom complet OTU domain containing 6B
Masse moléculaire calculée 293 aa, 34 kDa
Poids moléculaire observé 34-40 kDa
Numéro d’acquisition GenBankBC029760
Symbole du gène OTUD6B
Identification du gène (NCBI) 51633
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Protocole

Product Specific Protocols
WB protocol for OTUD6B antibody 25430-1-APDownload protocol
IHC protocol for OTUD6B antibody 25430-1-APDownload protocol
IF protocol for OTUD6B antibody 25430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Dorota (Verified Customer) (04-11-2019)

Antibody works perfectly for western blotting, however I could not optimise it for immunofluorescence. I tried series of dilutions and couple of different blocking/fixing methods, but there was no difference between knockdown and control conditions in any of them.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:500-1:5000
  • Cell Tissue Type: BT549
OTUD6B Antibody Western Blot,Immunofluorescence, validation (1:500-1:5000 dilution) in BT549 (Cat no:25430-1-AP)