Anticorps Polyclonal de lapin anti-OSBP

• Phare
• Validé par KD/KO

OSBP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 11096-1-AP

Synonymes

OSBP, OSBP1, oxysterol binding protein, Oxysterol binding protein 1

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Galerie de données de validation

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  WB analysis using 11096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11096-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 11096-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa cells using 11096-1-AP

  IP result of anti-OSBP (11096-1-AP for IP and Detection).

• 

  IHC staining of human ovary tumor using 11096-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11096-1-AP (OSBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 11096-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11096-1-AP (OSBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11096-1-AP cible OSBP dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, levure, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OSBP Protéine recombinante Ag1578
• Nom complet: oxysterol binding protein
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: BC011581
• Symbole du gène: OSBP
• Identification du gène (NCBI): 5007
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB,IF	Gastroenterology; Oxysterol-Binding Protein Is a Phosphatidylinositol 4-Kinase Effector Required for HCV Replication Membrane Integrity and Cholesterol Trafficking.; Authors - Hongliang Wang
human	WB	Cell Host Microbe; The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry.; Authors - Samad Amini-Bavil-Olyaee
human	WB,IF	PLoS Pathog; Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus.; Authors - Cristina M Dorobantu ; KD Validated
		Int J Mol Sci; A Small Molecule, 4-Phenylbutyric Acid, Suppresses HCV Replication via Epigenetically Induced Hepatic Hepcidin.; Authors - Kiyoon Kim
yeast	WB	Hum Mol Genet; Molecular partners of hNOT/ALG3, the human counterpart of the Drosophila NOT and yeast ALG3 gene, suggest its involvement in distinct cellular processes relevant to congenital disorders of glycosylation, cancer, neurodegeneration and a variety of further pathologies.; Authors - Benedikt Hacker

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (09-12-2019)

This antibody could detect endogenous OSBP protein both in WB and IF.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF