Anticorps Polyclonal de lapin anti-ORM1/2

ORM1/2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 11199-1-AP

Synonymes

AGP 2, AGP B, AGP B', AGP2, Alpha 1 acid glycoprotein 2, OMD 2, ORM1/2, ORM2, orosomucoid 2

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Galerie de données de validation

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  WB analysis using 11199-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11199-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11199-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11199-1-AP

  IP result of anti-ORM1/2 (IP:11199-1-AP, 3ug; Detection:11199-1-AP 1:800) with L02 cells lysate 2500ug.

• 

  IHC staining of human liver using 11199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11199-1-AP

  Immunofluorescent analysis of Hela cells, using ORM2 antibody 11199-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules A549, tissu hépatique de souris
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

11199-1-AP cible ORM1/2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ORM1/2 Protéine recombinante Ag1667
• Nom complet: orosomucoid 2
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24-28 kDa, 41-47 kDa
• Numéro d’acquisition GenBank: BC015964
• Symbole du gène: ORM2
• Identification du gène (NCBI): 5005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can recognize both ORM1 and ORM2.

Publications

Species	Application	Title
mouse		Carcinogenesis; FXR-Gankyrin axis is involved in development of pediatric liver cancer.; Authors - Leila Valanejad
mouse	WB	Mol Cell Biol; RNA Binding Protein CUGBP1 Inhibits Liver Cancer in a Phosphorylation-Dependent Manner.; Authors - Kyle Lewis
human	WB	BMC Cancer; Differential profiling of breast cancer plasma proteome by isotope-coded affinity tagging method reveals biotinidase as a breast cancer biomarker.; Authors - Kang Un-Beom UB

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hanyang (Verified Customer) (03-03-2022)

Considering results we got, the capacity of antibody is regarded as suspicious.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:400

ORM1/2 Antibody Immunohistochemistry,Immunofluorescence validation (1:400 dilution) in (Cat no:11199-1-AP)

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