Anticorps Polyclonal de lapin anti-ORM1

• Phare
• Validé par KD/KO

ORM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (2)

N° de cat : 16439-1-AP

Synonymes

ORM1/2, ORM, OMD 1, Alpha-1-acid glycoprotein 1, Alpha 1 acid glycoprotein 1

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Galerie de données de validation

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  WB analysis using 16439-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16439-1-AP (ORM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 16439-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 16439-1-AP (ORM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 16439-1-AP

  IP result of anti-ORM1 (IP:16439-1-AP, 3ug; Detection:16439-1-AP 1:300) with human plasma lysate 100ug.

• 

  IHC staining of human liver using 16439-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16439-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16439-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16439-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16439-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ORM1 antibody (16439-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16439-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ORM1 Polyclonal antibody (16439-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu vésical de souris
• Résultats positifs en IP: tissu plasmatique humain
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

16439-1-AP cible ORM1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ORM1 Protéine recombinante Ag9758
• Nom complet: orosomucoid 1
• Masse moléculaire calculée: 201 aa, 24 kDa
• Poids moléculaire observé: 40-47 kDa
• Numéro d’acquisition GenBank: BC026238
• Symbole du gène: ORM1
• Identification du gène (NCBI): 5004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein.

Publications

Species	Application	Title
mouse	WB	Nat Commun; The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation.; Authors - Mehrpouya B Mobin
human	WB,IHC	Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
mouse		Carcinogenesis; FXR-Gankyrin axis is involved in development of pediatric liver cancer.; Authors - Leila Valanejad
mouse	WB	Sci Rep; An acute phase protein α1-acid glycoprotein mitigates AKI and its progression to CKD through its anti-inflammatory action.; Authors - Hiroshi Watanabe
human	WB	Diagnostics (Basel); Urine Proteome in Distinguishing Hepatic Steatosis in Patients with Metabolic-Associated Fatty Liver Disease.; Authors - Chang-Hai Liu
mouse	WB,IF	Biochem Biophys Res Commun; Thapsigargin suppresses alpha 1-acid glycoprotein secretion independently of N-glycosylation and ER stress.; Authors - Nanami Goto

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SONAM (Verified Customer) (10-24-2023)

Antibody worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cells

FH

Chandrima (Verified Customer) (10-24-2023)

Antibody works really well. No non-specific binding

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cells

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C