Anticorps Polyclonal de lapin anti-OMA1

• Phare
• Validé par KD/KO

OMA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 17116-1-AP

Synonymes

2010001O09Rik, DAB1, MPRP 1, MPRP1, OMA1, YKR087C, ZMPOMA1

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Galerie de données de validation

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  WB analysis using 17116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17116-1-AP (OMA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17116-1-AP (OMA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17116-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17116-1-AP (OMA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17116-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17116-1-AP (OMA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17116-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17116-1-AP (OMA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules HepG2, cellules K-562, cellules MDA-MB-231, cellules SKOV-3
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 21 publications below

Informations sur le produit

17116-1-AP cible OMA1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OMA1 Protéine recombinante Ag10803
• Nom complet: OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
• Masse moléculaire calculée: 524 aa, 60 kDa
• Poids moléculaire observé: 34, 60 kDa
• Numéro d’acquisition GenBank: BC012915
• Symbole du gène: OMA1
• Identification du gène (NCBI): 115209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OMA1 (Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1 (Metalloprotease-related protein 1) and belongs to the peptidase M48 family. It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants, the 40 kDa form of OMA1 may be the mature form (M-OMA1), and the 35 kDa is a cleaved form (S-OMA1), with the 60 kDa form being the active enzyme (PMID:20334834, 30518622). This antibody is speicific to OMA1.

Publications

Species	Application	Title
human	WB	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
human	WB	Leukemia; DELE1 haploinsufficiency causes resistance to mitochondrial stress-induced apoptosis in monosomy 5/del(5q) AML; Authors - Jean-François Spinella
mouse	WB	Redox Biol; Peroxiredoxin 3 deficiency induces cardiac hypertrophy and dysfunction by impaired mitochondrial quality control.; Authors - Seong Keun Sonn ; KD Validated
human	WB	Cell Death Dis; Inhibition of the mitochondria-shaping protein Opa1 restores sensitivity to Gefitinib in a lung adenocarcinomaresistant cell line; Authors - Masafumi Noguchi
human	WB	Cell Rep; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria; Authors - Shiori Akabane