Anticorps Polyclonal de lapin anti-hIST1

hIST1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 19842-1-AP

Synonymes

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

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Galerie de données de validation

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  WB analysis using 19842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19842-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19842-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19842-1-AP

  IP result of anti-hIST1 (IP:19842-1-AP, 4ug; Detection:19842-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human hepatocirrhosis using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 19842-1-AP

  Immunofluorescent analysis of HepG2 cells using 19842-1-AP (hIST1 antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 5 publications below
• RIP: See 1 publications below

Informations sur le produit

19842-1-AP cible hIST1 dans les applications de WB, IHC, IF/ICC, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: hIST1 Protéine recombinante Ag13874
• Nom complet: KIAA0174
• Masse moléculaire calculée: 364 aa, 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC000116
• Symbole du gène: hIST1
• Identification du gène (NCBI): 9798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB	Dev Cell; NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.; Authors - Oliver B Davis
human		MBio; Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.; Authors - Ekansh Mittal
mouse	WB,IF,RIP	J Neurochem; Reduced circ_lrrc49 in trigeminal ganglion contributes to neuropathic pain in mice by downregulating Ist1 and impairing autophagy; Authors - Qingfeng Tang
human,mouse	IF	Cell Rep; Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during antigen cross-presentation; Authors - Marine Gros
human	IF	bioRxiv; IST1 regulates select endosomal recycling pathways; Authors - Amy K Clippinger