Anticorps Polyclonal de lapin anti-OFD1

OFD1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (2)

N° de cat : 22851-1-AP

Synonymes

71 7A, CXorf5, OFD1, oral facial digital syndrome 1, Protein 71 7A, SGBS2

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Galerie de données de validation

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  WB analysis of HeLa using 22851-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22851-1-AP (OFD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 22851-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22851-1-AP (OFD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 22851-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22851-1-AP (OFD1 Antibody) at dilution of 1:50. (under 10x lens).

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  IHC staining of human brain using 22851-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22851-1-AP (OFD1 Antibody) at dilution of 1:50. (under 40x lens).

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  IF Staining of HEK-293 using 22851-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using OFD1 antibody (22851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

22851-1-AP cible OFD1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OFD1 Protéine recombinante Ag18898
• Nom complet: oral-facial-digital syndrome 1
• Masse moléculaire calculée: 1012 aa, 117 kDa
• Poids moléculaire observé: 43 kDa, 120 kDa
• Numéro d’acquisition GenBank: BC096344
• Symbole du gène: OFD1
• Identification du gène (NCBI): 8481
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OFD1, also named CXorf5 and 71-7A, belongs to the OFD1 family. It has been implicated in several developmental syndromes, including a male-lethal X-linked dominant condition, Oral-Facial-Digital type 1 (OFD1) syndrome, X-linked recessiveSimpson-Golabi-Behmel syndrome type 2 (SGBS2), and Joubert syndrome and related disorders (JSRDs). It is a component of the centrioles controlling mother and daughter centrioles' length. It recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. OFD1 plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. This antibody recognizes all the isoforms of OFD1. The 43 kDa is isoform 2. The ~80 kDa band is unknown.

Publications

Species	Application	Title
human	IF	Nat Commun; Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.; Authors - Dragomir B Krastev
human	IF	PLoS Biol; Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.; Authors - Özge Z Aydin
rat	WB	Biochem Biophys Res Commun; BIX-01294-induced autophagy regulates elongation of primary cilia.; Authors - Ji Hyun Shin
human	WB,IF	Oncol Lett; Abnormal accumulation of OFD1 in endometrial cancer with poor prognosis inhibits ciliogenesis.; Authors - Ryuji Kojima
human,mouse	WB	Cell Death Dis; PMID: 31844040; Authors - Ji-Eun Bae
zebrafish	WB	Cell Biosci; IK is essentially involved in ciliogenesis as an upstream regulator of oral-facial-digital syndrome ciliopathy gene, ofd1; Authors - Hye In Ka

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (06-15-2022)

HEK293 cells stained for Hoechst (DNA marker, in green), OFD1 (mother centriole distal appendage marker, in magenta) and PCNT (pericentriolar matrix marker, in green). HEK293 cells were plated on Poly-lysine coated coverslips and fixed in cold methanol for 2' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). OFD1 recognises mother centriole.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: HEK293 cells (Human embryonic kidney 293 cells)

OFD1 Antibody Immunofluorescence validation (1:300 dilution) in HEK293 cells (Human embryonic kidney 293 cells) (Cat no:22851-1-AP)

FH

Sarah (Verified Customer) (02-16-2021)

Works very well for detection of OFD1 in HEK 293T cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: HEK 293T

OFD1 Antibody Immunofluorescence validation (1:300 dilution) in HEK 293T (Cat no:22851-1-AP)