Anticorps Polyclonal de lapin anti-ODC1

ODC1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 23454-1-AP

Synonymes

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

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Galerie de données de validation

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  WB analysis of RAW264.7 using 23454-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23454-1-AP (ODC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 23454-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23454-1-AP (ODC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules RAW264.7
• Résultats positifs en IHC: tissu d'hyperplasie de la prostate humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

23454-1-AP cible ODC1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ODC1 Protéine recombinante Ag10927
• Nom complet: ornithine decarboxylase 1
• Masse moléculaire calculée: 461 aa, 51 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC025296
• Symbole du gène: ODC1
• Identification du gène (NCBI): 4953
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.