Anticorps Polyclonal de lapin anti-OBFC2A

• Phare
• Validé par KD/KO

OBFC2A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16719-1-AP

Synonymes

hSSB2, OBFC2A, Sensor of ssDNA subunit B2, SOSS B2, SOSS complex subunit B2, SSB2

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Galerie de données de validation

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  WB analysis of COLO 320 using 16719-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16719-1-AP (OBFC2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16719-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16719-1-AP (OBFC2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules COLO 320

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

16719-1-AP cible OBFC2A dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OBFC2A Protéine recombinante Ag10158
• Nom complet: oligonucleotide/oligosaccharide-binding fold containing 2A
• Masse moléculaire calculée: 124 aa, 14 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC107723
• Symbole du gène: OBFC2A
• Identification du gène (NCBI): 64859
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Single-stranded DNA (ssDNA)-binding proteins (SSBs) are ubiquitous and essential for a variety of DNA metabolic processes, including recombination, replication, and detection and repair of damage. SSBs have multiple roles in binding and sequestering ssDNA, detecting DNA damage, stimulating nucleases, helicases and strand-exchange proteins, activating transcription and mediating protein-protein interactions [PMID:18449195] Human single-stranded DNA-binding protein 1 (hSSB1), encoded by OBFC2B, is characterized as an essential factor for the initiation of DNA damage checkpoints and the maintenance of genomic stability [PMID:22940690].

Publications

Species	Application	Title
mouse	WB	Blood; Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress.; Authors - Wei Shi
human	WB	Cell Res; The Integrator complex controls the termination of transcription at diverse classes of gene targets.; Authors - Jeffrey R Skaar ; KD Validated
mouse	WB	EMBO J; The hSSB1 orthologue Obfc2b is essential for skeletogenesis but dispensable for the DNA damage response in vivo.; Authors - Feldhahn Niklas N ; KD Validated
human	WB	Front Oncol; The Potential Prognostic Role of Oligosaccharide-Binding Fold-Containing Protein 2A (OBFC2A) in Triple-Negative Breast Cancer.; Authors - Qianxue Wu
mouse	WB	J Immunol; SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors.; Authors - Matthias Pfeifer ; KO Validated
human,mouse	IP	Sci Rep; hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage.; Authors - Didier Boucher