Anticorps Polyclonal de lapin anti-OAS3

• Phare
• Validé par KD/KO

OAS3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 21915-1-AP

Synonymes

(2 5)oligo(A) synthase 3, 2 5 oligoadenylate synthase 3, 2 5A synthase 3, OAS3, p100, p100 OAS, p100OAS

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Galerie de données de validation

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  WB analysis using 21915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21915-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 21915-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 21915-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21915-1-AP

  Immunofluorescent analysis of HeLa cells using 21915-1-AP (OAS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HeLa, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below

Informations sur le produit

21915-1-AP cible OAS3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OAS3 Protéine recombinante Ag16150
• Nom complet: 2'-5'-oligoadenylate synthetase 3, 100kDa
• Masse moléculaire calculée: 1087 aa, 121 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC113746
• Symbole du gène: OAS3
• Identification du gène (NCBI): 4940
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS3, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). It is also named as p100 OAS and belongs to the 2-5A synthase family. OAS3 is involved in inhibition of cellular protein synthesis and in viral infection resistance. This antibody may have weak cross-reaction with OAS1 protein due to the highly homology.

Publications

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen ; KO Validated
human	WB	Nat Cell Biol; Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes; Authors - Dario F De Jesus
human		Nat Commun; Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.; Authors - Wei Liu
human	WB	J Invest Dermatol; OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.; Authors - Yan-Zhou Huang ; KD Validated
human	WB	Virol Sin; EV71 3C protease cleaves host anti-viral factor OAS3 and enhances virus replication.; Authors - Xiaolei Zhou ; KD Validated
human	WB	Virol Sin; IFN-β1b induces OAS3 to inhibit EV71 via IFN-β1b/JAK/STAT1 pathway; Authors - Baisong Zheng