Anticorps Polyclonal de lapin anti-OAS2

OAS2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 14214-1-AP

Synonymes

(2 5)oligo(A) synthase 2, 2 5 oligoadenylate synthase 2, 2 5A synthase 2, OAS2, p69 OAS / p71 OAS, p69OAS / p71OAS

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Galerie de données de validation

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  WB analysis of Jurkat using 14214-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14214-1-AP (OAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14214-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14214-1-AP (OAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14214-1-AP (OAS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14214-1-AP (OAS2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14214-1-AP cible OAS2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OAS2 Protéine recombinante Ag5436
• Nom complet: 2'-5'-oligoadenylate synthetase 2, 69/71kDa
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 66-71 kDa
• Numéro d’acquisition GenBank: BC049215
• Symbole du gène: OAS2
• Identification du gène (NCBI): 4939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS/p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody recognizes isoform p71 and isoform p69 of OAS2.