Anticorps Polyclonal de lapin anti-NVL

NVL Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 16970-1-AP

Synonymes

nuclear VCP like, Nuclear VCP like protein, NVL, NVLp

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Galerie de données de validation

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  WB analysis using 16970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16970-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16970-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16970-1-AP

  Immunofluorescent analysis of HepG2 cells using 16970-1-AP (NVL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules HeLa, tissu de thymus de rat, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

16970-1-AP cible NVL dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NVL Protéine recombinante Ag9205
• Nom complet: nuclear VCP-like
• Masse moléculaire calculée: 856aa,95 kDa; 659aa,73 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC012105
• Symbole du gène: NVL
• Identification du gène (NCBI): 4931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nature; Systematic quantitative analysis of ribosome inventory during nutrient stress.; Authors - Heeseon An
human	IF	J Cell Biol; Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases.; Authors - Yun Chawon C
human	WB,IF	Nat Commun; Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins; Authors - Arthur Louche

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (04-05-2020)

This antibody works okay. There are a few nonspecific bands, but I could tell which is the target band because I performed nuclear cytosolic fractionation. NVL is supposed to be in both nucleus and cytosol to shuttle ribosomes.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

NVL Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:16970-1-AP)