Anticorps Polyclonal de lapin anti-NUPR1

• Phare
• Validé par KD/KO

NUPR1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 15056-1-AP

Synonymes

Candidate of metastasis 1, COM1, nuclear protein 1, NUPR1, P8, Protein p8

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Galerie de données de validation

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  WB analysis of L02 using 15056-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15056-1-AP (NUPR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15056-1-AP (NUPR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15056-1-AP (NUPR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules L02
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la prostate humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:600
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

15056-1-AP cible NUPR1 dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUPR1 Protéine recombinante Ag7039
• Nom complet: nuclear protein 1
• Masse moléculaire calculée: 8 kDa
• Poids moléculaire observé: 8 kDa, 17 kDa, 38 kDa
• Numéro d’acquisition GenBank: BC002434
• Symbole du gène: NUPR1
• Identification du gène (NCBI): 26471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. The predicted MW of NUPR1 is 8 kDa and we also detectet bands around 17 and 38 kDa, which are similar to the papers(PMID: 29130426, 27336713, 28694771, 22961798). NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation (PMID: 33542201), and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins (PMID: 36858171).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,CoIP	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan ; KD Validated
human	WB	J Inorg Biochem; Chronic nickel exposure alters extracellular vesicles to mediate cancer progression via sustained NUPR1 expression; Authors - Shan Liu ; KD Validated
human	WB	PLoS One; Adenomatous Polyposis Coli loss controls cell cycle regulators and response to paclitaxel in MDA-MB-157 metaplastic breast cancer cells.; Authors - Emily M Astarita
human,mouse	IHC,CoIP	Cell Death Discov; The interplay of transcriptional coregulator NUPR1 with SREBP1 promotes hepatocellular carcinoma progression via upregulation of lipogenesis; Authors - Yongjia Wang ; KO Validated
human	WB,IHC	Cancer Med; NUPR1 imparts oncogenic potential in bladder cancer; Authors - Lifeng Zhang ; KD Validated
human	WB	PPAR Res; Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma; Authors - Chao Lu