• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-NUFIP1

NUFIP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 12515-1-AP

Synonymes

NUFIP, NUFIP1



Applications testées

Résultats positifs en WBcellules HeLa, cellules A375
Résultats positifs en IPcellules HeLa
Résultats positifs en IHCtissu de tumeur ovarienne humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

12515-1-AP cible NUFIP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène NUFIP1 Protéine recombinante Ag3197
Nom complet nuclear fragile X mental retardation protein interacting protein 1
Masse moléculaire calculée 56 kDa
Poids moléculaire observé 70-75 kDa
Numéro d’acquisition GenBankBC017745
Symbole du gène NUFIP1
Identification du gène (NCBI) 26747
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of FMRP (Fragile X Mental Retardation Protein). FMRP is an RNA binding protein reported to be involved in translational control, notably at postsynaptic sites of protein synthesis as a part of a multiprotein/mRNA complex[PMID:12941608]. NUFIP1 is one of the several FMRP-interacting proteins. NUFIP can act as a pol II-specific basal transcriptional activator in vitro and when ectopically overexpressed in vivo. NUFIP can directly activate promoters by enhancing the ATP-dependent release of hyperphosphorylated form of pol II from open transcription complexes[PMID:15107825].

Protocole

Product Specific Protocols
WB protocol for NUFIP1 antibody 12515-1-APDownload protocol
IHC protocol for NUFIP1 antibody 12515-1-APDownload protocol
IF protocol for NUFIP1 antibody 12515-1-APDownload protocol
IP protocol for NUFIP1 antibody 12515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Systematic quantitative analysis of ribosome inventory during nutrient stress.

Authors - Heeseon An
  • KO Validated
humanWB

Science

NUFIP1 is a ribosome receptor for starvation-induced ribophagy.

Authors - Gregory A Wyant
  • KO Validated
mouseWB

Sci Adv

The 40S-LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity.

Authors - Pedro Fuentes
  • KD Validated
humanWB,IF

Autophagy

The autophagic protein LC3 translocates to the nucleus and localizes in the nucleolus associated to NUFIP1 in response to cyclic mechanical stress.

Authors - Myoung Sup Shim
humanWB

J Cell Biol

Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.

Authors - Jonathan Bizarro
humanWB

Nucleic Acids Res

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Authors - Anne-Sophie Gribling-Burrer

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

H (Verified Customer) (04-06-2020)

This antibody is okay, but there are multiple off-target bands especially in the nucleus. I have performed immunofluorescence analysis using KO cells, and I found significant signal in the nucleus even in the KO cells, requiring caution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T, HEK293 and HCT116
NUFIP1 Antibody Western Blot validation (1:1000 dilution) in HEK293T, HEK293 and HCT116 (Cat no:12515-1-AP)