Anticorps Polyclonal de lapin anti-NUDT3/4/10/11

NUDT3/4/10/11 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 10912-1-AP

Synonymes

APS1, ASP1, DIPP 3 beta, DIPP3 beta, DIPP3b, DIPP3beta, hAps1, hDIPP3beta, Nudix motif 11, NUDT11

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Galerie de données de validation

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  WB analysis using 10912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10912-1-AP (NUDT11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10912-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10912-1-AP (NUDT11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10912-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10912-1-AP (NUDT11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10912-1-AP cible NUDT3/4/10/11 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUDT3/4/10/11 Protéine recombinante Ag1346
• Nom complet: nudix (nucleoside diphosphate linked moiety X)-type motif 11
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC009942
• Symbole du gène: NUDT11
• Identification du gène (NCBI): 55190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUDT11 (nucleoside diphosphate linked moiety X-type motif 11), also named as DIPP3B or APS1, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).