Anticorps Polyclonal de lapin anti-NUDT3/4/10/11

NUDT3/4/10/11 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 17508-1-AP

Synonymes

APS2, DIPP 3 alpha, DIPP3 alpha, DIPP3a, hAps2, hDIPP3alpha, Nudix motif 10, NUDT10

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Galerie de données de validation

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  WB analysis using 17508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17508-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17508-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17508-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17508-1-AP

  Immunofluorescent analysis of Hela cells, using NUDT10 antibody 17508-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

17508-1-AP cible NUDT3/4/10/11 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUDT3/4/10/11 Protéine recombinante Ag11711
• Nom complet: nudix (nucleoside diphosphate linked moiety X)-type motif 10
• Masse moléculaire calculée: 164 aa, 19 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC050700
• Symbole du gène: NUDT10
• Identification du gène (NCBI): 170685
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUDT10 (nucleoside diphosphate linked moiety X-type motif 10), also named as DIPP3A or APS2, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).