Anticorps Polyclonal de lapin anti-NUDC

• Phare
• Validé par KD/KO

NUDC Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10681-1-AP

Synonymes

HNUDC, MNUDC, Nuclear migration protein nudC, NUDC

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Galerie de données de validation

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  WB analysis of HepG2 using 10681-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10681-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10681-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10681-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10681-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10681-1-AP

  IP result of anti-NUDC (IP:10681-1-AP, 4ug; Detection:10681-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human pancreas cancer using 10681-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10681-1-AP (NUDC antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 10681-1-AP

  Immunofluorescent analysis of HeLa cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10681-1-AP

  Immunofluorescent analysis of HepG2 cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10681-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, cellules K-562, cellules SH-SY5Y
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HEK-293, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10681-1-AP cible NUDC dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUDC Protéine recombinante Ag0998
• Nom complet: nuclear distribution gene C homolog (A. nidulans)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: BC006147
• Symbole du gène: NUDC
• Identification du gène (NCBI): 10726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear distribution protein C (NudC) is a highly conserved dynein/dynactin associated protein implicated in mitosis and cytokinesis. NudC is localized on major mitotic structures, and is involved in regulating spindle formation stability of kinetochore microtubule attachments, and chromosomes congression in early mitosis. NudC also is required for multi-protein transport along axons in neurons.

Publications

Species	Application	Title
human	WB,IF	Cell Res; NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.; Authors - Cheng Zhang ; KD Validated
human	IF	Int J Mol Sci; N-Acetyl-D-Glucosamine Kinase Interacts with NudC and Lis1 in Dynein Motor Complex and Promotes Cell Migration.; Authors - Md Ariful Islam
human	WB	Mol Cell Biol; Substrate Trapping Proteomics Reveals Targets of the βTrCP2/FBXW11 Ubiquitin Ligase.; Authors - Tai Young Kim
human	WB	Front Cell Dev Biol; NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration.; Authors - Min Liu
mouse	WB,IHC	Reprod Domest Anim; Proteomic-based identification of oocyte maturation-related proteins in mouse germinal vesicle oocytes.; Authors - Senyang Cao