Anticorps Polyclonal de lapin anti-NUAK2

• Phare
• Validé par KD/KO

NUAK2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11592-1-AP

Synonymes

NUAK family SNF1 like kinase 2, NUAK2, SNARK, SNF1/AMP kinase related kinase

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Galerie de données de validation

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  WB analysis of Y79 using 11592-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11592-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11592-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 11592-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 11592-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 11592-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11592-1-AP (NUAK2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 11592-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11592-1-AP (NUAK2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH3T3 cells using 11592-1-AP

  NIH3T3 cells on cover slips fixed and staining with anti-NUAK2 (11592-1-AP,1:100;Rabbit polyclonal -Red) and anti-pericentrin(centrosome Marker; McAb-Green). The photo shows the centrosome in cell cycle: Interphase, Prophase, Prometaphase, Metaphase, Anaphase and Telophase. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml.(from Dr. Zineldeen Doaa Hussein) .

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

11592-1-AP cible NUAK2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUAK2 Protéine recombinante Ag2031
• Nom complet: NUAK family, SNF1-like kinase, 2
• Masse moléculaire calculée: 628 aa, 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC017306
• Symbole du gène: NUAK2
• Identification du gène (NCBI): 81788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUAK2(NUAK family SNF1-like kinase 2) is also named as OMPHK2, SNARK and belongs to the SNF1 subfamily. The function of NUAK2 is partly dependent on anti-apoptotic properties against apoptosis induced by death ligand and NUAK2 also has effects on the migration of cancer cells as speculated from studies on myosin filaments and cytoskeleton organization in normal cells(PMID:21911917). Western blot analysis detected NUAK2 expressed in cancer cell lines of diverse tissue origin(PMID:12409306).

Publications

Species	Application	Title
human	WB,IHC	Proc Natl Acad Sci U S A; AMP kinase-related kinase NUAK2 affects tumor growth, migration, and clinical outcome of human melanoma.; Authors - Namiki Takeshi T
human	WB, IF	Cancer Res; NUAK2 amplification coupled with PTEN deficiency promote melanoma development via CDK activation.; Authors - Takeshi Namiki ; KD Validated
human	WB	Diabetologia; Regulation of skeletal muscle sucrose, non-fermenting 1/AMP-activated protein kinase-related kinase (SNARK) by metabolic stress and diabetes.; Authors - Rune A A ; KD Validated
human	WB, IF	Ann Clin Biochem; Sucrose non-fermenting AMPK related kinase/Pentraxin 3 and DNA damage axis: a gateway to cardiovascular disease in systemic lupus erythematosus among Egyptian patients.; Authors - Doaa Hussein Zineldeen