Anticorps Polyclonal de lapin anti-NTN1-Specific

NTN1-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 20235-1-AP

Synonymes

netrin 1, NTN1, NTN1L, NTN1-Specific

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Galerie de données de validation

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  WB analysis using 20235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20235-1-AP (NTN1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20235-1-AP (NTN1-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 20235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20235-1-AP (NTN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20235-1-AP (NTN1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cérébral de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

20235-1-AP cible NTN1-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: netrin 1
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 65-68 kDa
• Numéro d’acquisition GenBank: NM_004822
• Symbole du gène: NTN1
• Identification du gène (NCBI): 9423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NTN1 is also named as NTN1L. Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. NTN1 is involved in colorectal tumorigenesis by regulating apoptosis. 20235-1-AP antibody detects 65-68 kDa (3 kDa signal peptide) protein in SDS-PAGE. This antibody is specific to NTN1.

Publications

Species	Application	Title
human	WB	J Diabetes Res; High-Glucose-Induced Injury to Proximal Tubules of the Renal System Is Alleviated by Netrin-1 Suppression of Akt/mTOR; Authors - Chenxiao Liu
human	WB	Int J Oncol; Netrin‑1 interference potentiates epithelial‑to‑mesenchymal transition through the PI3K/AKT pathway under the hypoxic microenvironment conditions of non‑small cell lung cancer.; Authors - Xiayun Jin
human	WB,IHC	J Int Med Res; Detection of netrin-1 as a novel biomarker for diagnosis and chemotherapeutic monitoring of lung cancer.; Authors - Yuanyuan Zhao
human,mouse	WB	Tissue Cell; Exosomes derived from human umbilical cord mesenchymal stem cells loaded with RVG-Lamp2b and Netrin-1 promotes Schwann cell invasion and migration; Authors - Jie He
mouse	WB	J Ethnopharmacol; Novel mechanisms of Anshen Dingzhi prescription against PTSD: Inhibiting DCC to modulate synaptic function and inflammatory responses; Authors - Jiamin Hu