Anticorps Polyclonal de lapin anti-NT5C2

• Phare
• Validé par KD/KO

NT5C2 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15223-1-AP

Synonymes

5' nucleotidase, cytosolic II, cN II, Cytosolic 5 nucleotidase II, GMP, NT5B, NT5C2, NT5CP, PNT5

---

Galerie de données de validation

• 

  WB analysis using 15223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15223-1-AP

  WB result of NT5C2 antibody (15223-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5C2 transfected A549 cells.

• 

  WB analysis of human placenta using 15223-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15223-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NT5C2 antibody (15223-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules NCI-H1299, cellules NCL-H1299, cellules NIH/3T3, tissu hépatique de rat, tissu hépatique de souris, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

15223-1-AP cible NT5C2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NT5C2 Protéine recombinante Ag7438
• Nom complet: 5'-nucleotidase, cytosolic II
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC001595
• Symbole du gène: NT5C2
• Identification du gène (NCBI): 22978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	BMC Med Genomics; Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.; Authors - Xing-Cheng Zhao
mouse	WB,IHC	Cell Death Dis; Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation; Authors - Mei-Xin Li