Anticorps Polyclonal de lapin anti-NSDHL

• Phare
• Validé par KD/KO

NSDHL Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 15111-1-AP

Synonymes

H105E3, NSDHL, Protein H105e3, SDR31E1, XAP104

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Galerie de données de validation

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  WB analysis of HeLa using 15111-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15111-1-AP (NSDHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15111-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15111-1-AP (NSDHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15111-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15111-1-AP (NSDHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 15111-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15111-1-AP (NSDHL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cardiaque humain, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu de cancer de l'estomac humain, tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de côlon humain, tissu de tumeur ovarienne humain, tissu d'estomac humain, tissu ovarien humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3200
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 6 publications below

Informations sur le produit

15111-1-AP cible NSDHL dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NSDHL Protéine recombinante Ag7097
• Nom complet: NAD(P) dependent steroid dehydrogenase-like
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa, 40 kDa
• Numéro d’acquisition GenBank: BC000245
• Symbole du gène: NSDHL
• Identification du gène (NCBI): 50814
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NSDHL(Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating) encodes a sterol dehydrogenase or decarboxylase involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis. Mutations in this gene are associated with human CHILD syndrome (congenital hemidysplasia with ichthyosiform nevus and limb defects), an X-linked, male lethal disorder, as well as the mouse mutations bare patches and striated.(PMID: 14506130).

Publications

Species	Application	Title
mouse	WB	Cancer Cell; Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer.; Authors - Linara Gabitova-Cornell ; KO Validated
mouse	IHC	Cancer Discov; Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation.; Authors - Sukhanova Anna A
mouse	WB,IHC	Clin Cancer Res; Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.; Authors - Renata E Gordon ; KD Validated
mouse	WB, IHC	Cell Rep; Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR.; Authors - Linara Gabitova
mouse	IHC	Cell Cycle; Insm2 deficiency results in female infertility by disturbing steroid pathway and decreasing ovarian reserve in mice.; Authors - Zhi-Ming Li
human	IHC	Mol Cancer Ther; EGFR and RB1 as Dual Biomarkers in HPV-Negative Head and Neck Cancer.; Authors - Tim N Beck