Anticorps Polyclonal de lapin anti-GABPA

• Phare
• Validé par KD/KO

GABPA Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 21542-1-AP

Synonymes

E4TF1 60, E4TF1A, GA binding protein alpha chain, GABP subunit alpha, GABPA, NFT2, Transcription factor E4TF1 60

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Galerie de données de validation

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  WB analysis using 21542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21542-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 21542-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21542-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21542-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21542-1-AP

  IP result of anti-GABPA (IP:21542-1-AP, 4ug; Detection:21542-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 21542-1-AP

  IP result of anti-GABPA (IP:21542-1-AP, 4ug; Detection:21542-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human breast cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21542-1-AP (GABPA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21542-1-AP (GABPA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21542-1-AP (GABPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21542-1-AP (GABPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 21542-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GABPA antibody (21542-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules A549, cellules K-562, cellules MCF-7, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

21542-1-AP cible GABPA dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GABPA Protéine recombinante Ag16191
• Nom complet: GA binding protein transcription factor, alpha subunit 60kDa
• Masse moléculaire calculée: 454 aa, 51 kDa
• Poids moléculaire observé: 56-60 kDa
• Numéro d’acquisition GenBank: BC035031
• Symbole du gène: GABPA
• Identification du gène (NCBI): 2551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Publications

Species	Application	Title
		Hepatology; High mobility group protein B1 controls liver cancer initiation through yes-associated protein -dependent aerobic glycolysis.; Authors - Ruochan Chen
human, mouse	WB,IHC,IF	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H ; KD Validated
mouse	WB	Cardiovasc Res; Sex differences in exercise-induced physiological myocardial hypertrophy are modulated by oestrogen receptor beta.; Authors - Elke Dworatzek
mouse	WB	Stem Cells; MZF1 and GABP Cooperate with Sox2 in Regulating the Expression of YAP1 in Cancer Cells.; Authors - Narendra Kumar Verma ; KD Validated
human	WB	Biology (Basel); TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.; Authors - Yanbing Wang
human	WB,IHC	BMC Cancer; GABPA predicts prognosis and inhibits metastasis of hepatocellular carcinoma.; Authors - Sheng Zhang ; KD Validated