Anticorps Monoclonal anti-NRCAM

NRCAM Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2E1C3

N° de cat : 68291-1-Ig

Synonymes

hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

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Galerie de données de validation

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  WB analysis using 68291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat cerebellum using 68291-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 68291-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 68291-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRCAM antibody (68291-1-Ig, Clone: 2E1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de porc, tissu cérébral de souris, tissu de cervelet de porc, tissu de cervelet de rat
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68291-1-Ig cible NRCAM dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NRCAM Protéine recombinante Ag20558
• Nom complet: neuronal cell adhesion molecule
• Masse moléculaire calculée: 1304 aa, 144 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC098401
• Symbole du gène: NRCAM
• Identification du gène (NCBI): 4897
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).