Anticorps Polyclonal de lapin anti-NRCAM

• Phare
• Validé par KD/KO

NRCAM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 21608-1-AP

Synonymes

Ng-CAM-related, Ng CAM related, Neuronal surface protein Bravo, neuronal cell adhesion molecule, KIAA0343

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Galerie de données de validation

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  WB analysis using 21608-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21608-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21608-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP (NRCAM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP (NRCAM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21608-1-AP (NRCAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21608-1-AP (NRCAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 21608-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 21608-1-AP (NRCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

21608-1-AP cible NRCAM dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NRCAM Protéine recombinante Ag16237
• Nom complet: neuronal cell adhesion molecule
• Masse moléculaire calculée: 1304 aa, 144 kDa
• Poids moléculaire observé: 130-136 kDa
• Numéro d’acquisition GenBank: BC098401
• Symbole du gène: NRCAM
• Identification du gène (NCBI): 4897
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).

Publications

Species	Application	Title
human	IF	Stem Cell Reports; CD73 Regulates Stemness and Epithelial-Mesenchymal Transition in Ovarian Cancer-Initiating Cells.; Authors - Michela Lupia
human	WB	Mol Cell Proteomics; Application of mass spectrometry profiling to establish brusatol as an inhibitor of global protein synthesis.; Authors - Steffan Vartanian
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin
human	WB	Cancer Gene Ther; NrCAM secreted by endometrial stromal cells enhances the progestin sensitivity of endometrial cancer cells through epigenetic modulation of PRB.; Authors - Yali Cheng ; KD Validated
mouse	IHC	Mol Cell Neurosci; Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.; Authors - Zhen-Huang Chen