Anticorps Polyclonal de lapin anti-NR5A1

NR5A1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, FC (Intra), IP, ELISA, IF

Conjugaison

Non conjugué

Publications

13

N° de cat : 18658-1-AP

Synonymes

FTZF1, FTZ1, ELP, Adrenal 4-binding protein, Adrenal 4 binding protein

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Galerie de données de validation

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  WB analysis using 18658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18658-1-AP (NR5A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 18658-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18658-1-AP (NR5A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 18658-1-AP

  IP result of anti-NR5A1 (IP:18658-1-AP, 4ug; Detection:18658-1-AP 1:300) with A2780 cells lysate 960ug.

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  IHC staining of human ovary using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of rat testis using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18658-1-AP (NR5A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18658-1-AP (NR5A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  FC experiment of HepG2 using 18658-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NR5A1 (18658-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris, tissu ovarien de rat
• Résultats positifs en IP: cellules A2780
• Résultats positifs en IHC: tissu ovarien humain, tissu hépatique humain, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Informations sur le produit

18658-1-AP cible NR5A1 dans les applications de WB, IHC, FC (Intra), IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, souris, Ondatra zibethicus
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR5A1 Protéine recombinante Ag13252
• Nom complet: nuclear receptor subfamily 5, group A, member 1
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC032501
• Symbole du gène: NR5A1
• Identification du gène (NCBI): 2516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Steroidogenic factor-1 (SF-1,STF-1), also known as NR5A1, regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global and tissue-specific knockout mice have demonstrated that SF-1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with mutations that render SF-1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency. Furthermore, dysregulation of SF-1 has been linked to diseases such as endometriosis and adrenocortical carcinoma.Like other nuclear hormone receptors, SF-1 has a modular domain structure composed of an N-terminal zinc finger DNA-binding domain, a ligand-binding domain, a C-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. SF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain. SF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors. In addition to phosphorylation at Ser203, SF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses SF-1 function, acetylation enhances its transcriptional activity.In the adult ovary, SF-1 localizes to theca/interstitial cells.

Publications

Species	Application	Title
human	IF	Elife; Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.; Authors - Jianlin Liang
mouse	WB,IF	Elife; PRMT5 regulates ovarian follicle development by facilitating Wt1 translation; Authors - Min Chen
mouse	IHC	Mol Cell Endocrinol; Pde8b haploinsufficiency in mice is associated with modest adrenal defects, impaired steroidogenesis, and male infertility, unaltered by concurrent PKA or Wnt activation.; Authors - Leticia Ferro Leal
Ondatra zibethicus	IHC	J Steroid Biochem Mol Biol; Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus).; Authors - Wenqian Xie
mouse	IF	Toxicol Appl Pharmacol; PMID: 31175881; Authors - Jiandong Sun
mouse	WB	Toxicol Appl Pharmacol; Icariin promotes mouse Leydig cell testosterone synthesis via the Esr1/Src/Akt/Creb/Sf-1 pathway.; Authors - Jiandong Sun