Anticorps Polyclonal de lapin anti-NR4A1

• Phare
• Validé par KD/KO

NR4A1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

28

N° de cat : 12235-1-AP

Synonymes

Early response protein NAK1, GFRP1, HMR, N10, NAK 1, NAK1, NGFIB, NR4A1, NUR77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST 59, Testicular receptor 3, TR3

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Galerie de données de validation

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  WB analysis using 12235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12235-1-AP (NR4A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12235-1-AP (NR4A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12235-1-AP

  IP result of anti-NR4A1 (IP:12235-1-AP, 4ug; Detection:12235-1-AP 1:1000) with HeLa cells lysate 1720 ug.

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  IHC staining of human pancreas cancer using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 12235-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 12235-1-AP (NR4A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules L02, cellules PC-3, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de côlon de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 3 publications below

Informations sur le produit

12235-1-AP cible NR4A1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR4A1 Protéine recombinante Ag2875
• Nom complet: nuclear receptor subfamily 4, group A, member 1
• Masse moléculaire calculée: 598 aa, 64 kDa
• Poids moléculaire observé: 64-70 kDa
• Numéro d’acquisition GenBank: BC016147
• Symbole du gène: NR4A1
• Identification du gène (NCBI): 3164
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR4A1 is an orphan nuclear receptor that act concomitantly with NURR1 in regulating the expression of delay-early genes during liver regeneration. Also NR4A1 has linked not only to cell apoptosis but also proliferation in response to growth factor. It plays a role in caspase-independent cell death by activation of the ERK pathway and increased activity of MEF2 transcription factors. NR4A1 exists some isoforms with MW 65 and 34 kDa.

Publications

Species	Application	Title
mouse	IF	Aging (Albany NY); A systematic dissection of human primary osteoblasts in vivo at single-cell resolution.; Authors - Yun Gong
rat	WB	Sci Rep; Nur77 exacerbates PC12 cellular injury in vitro by aggravating mitochondrial impairment and endoplasmic reticulum stress.; Authors - Huimin Gao ; KD Validated
mouse	WB,IF	Sci Rep; NR4A1 Knockdown Suppresses Seizure Activity by Regulating Surface Expression of NR2B.; Authors - Yanke Zhang ; KD Validated
mouse	WB, IF	Mol Neurobiol; Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment.; Authors - Xiaobo Wei
human,mouse	WB	Bioorg Chem; Structure-based design and synthesis of anti-fibrotic compounds derived from para-positioned 3,4,5-trisubstituted benzene; Authors - Wenbin Hong
human	WB	Sci Rep; The E3 ubiquitin ligase Trim13 regulates Nur77 stability via casein kinase 2α.; Authors - Bin Huang