Anticorps Polyclonal de lapin anti-NR2F1

• Phare
• Validé par KD/KO

NR2F1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 24573-1-AP

Synonymes

COUP TF I, COUP TF1, COUP TFI, COUP transcription factor 1, COUP transcription factor I, EAR 3, EAR3, ERBAL3, NR2F1, NR2F2, SVP44, TCFCOUP1, TFCOUP1, V erbA related protein 3

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Galerie de données de validation

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  WB analysis of HEK-293 using 24573-1-AP

  WB result of NR2F1 antibody (24573-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR2F1 transfected HEK-293 cells.

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  WB analysis using 24573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24573-1-AP (NR2F1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24573-1-AP (NR2F1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24573-1-AP (NR2F1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24573-1-AP (NR2F1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24573-1-AP (NR2F1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules NIH/3T3, cellules PC-3
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

24573-1-AP cible NR2F1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR2F1 Protéine recombinante Ag0118
• Nom complet: nuclear receptor subfamily 2, group F, member 1
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC004154
• Symbole du gène: NR2F1
• Identification du gène (NCBI): 7025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR2F1 (Nuclear receptor subfamily 2, group F, member 1), also known as COUP-TFI, is an orphan nuclear receptor belonging to the superfamily of steroid/thyroid hormone receptors and acting as a strong transcriptional regulator. NR2F1 has a DNA binding domain (DBD) consisting of two conserved Zinc-finger motifs and a ligand-binding domain (LBD). NR2F1 also plays an epigenetic role in mediating global histone modifications by interacting with or recruiting chromatin-remodeling enzymes. NR2F1 can be a biomarker of tumor dormancy that promotes quiescence of various types of cancer cells, including breast, prostate, and squamous cell carcinoma of the head and neck (PMID: 35740627).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC	J Exp Clin Cancer Res; Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.; Authors - Xiao Yang
human	WB,IF	Cell Death Dis; Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.; Authors - Meng Zhang
human	WB,IHC	Cancers (Basel); NR2F1 Regulates TGF-β1-Mediated Epithelial-Mesenchymal Transition Affecting Platinum Sensitivity and Immune Response in Ovarian Cancer; Authors - Qiuju Liang ; KD Validated
human	WB	Clin Transl Med; Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma; Authors - Hao Zhang
human	WB	Transl Oncol; Hypoxia induced cell dormancy of salivary adenoid cystic carcinoma through miR-922/DEC2 axis; Authors - Li Dai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

NR2F1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:24573-1-AP)