Anticorps Polyclonal de lapin anti-PXR

• Phare
• Validé par KD/KO

PXR Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 15607-1-AP

Synonymes

BXR, NR1I2, ONR1, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, PAR, PAR1, PAR2, PARq, Pregnane X receptor, PRR, PXR, SAR, SXR

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Galerie de données de validation

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  WB analysis of MCF-7 using 15607-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15607-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15607-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 15607-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Applications testées

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15607-1-AP cible PXR dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PXR Protéine recombinante Ag7991
• Nom complet: nuclear receptor subfamily 1, group I, member 2
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC017304
• Symbole du gène: PXR
• Identification du gène (NCBI): 8856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, plays a key role in the induction of genes involved in drug transport and metabolism. It modulates drug transport and metabolism through the regulation of target genes responsible for the transport and conversion of chemicals into metabolites that are more easily eliminated from the body [PMID:22609277]. It is also emerging as an endobiotic receptor that regulates the inflammatory response. In addition, PXR acts as a transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds [PMID: 19297428].

Publications

Species	Application	Title
human	IF,WB	Hepatol Int; Hepatitis B virus-related intrahepatic cholangiocarcinoma originates from hepatocytes; Authors - Zimin Song
mouse	CoIP	Cell Death Dis; Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.; Authors - Cheng Wang ; KD Validated
human	WB	Biochem Pharmacol; Constitutive androstane receptor weakens the induction of panaxytriol on CYP3A4 by repressing the activation of pregnane X receptor.; Authors - Qingqing Hu
mouse	WB	Br J Nutr; Retinoic acid induces macrophage cholesterol efflux and inhibits atherosclerotic plaque formation in apoE-deficient mice.; Authors - Wenjing Zhou
human	WB,IHC	Ann Transl Med; Curcuma zedoaria petroleum ether extract reverses the resistance of triple-negative breast cancer to docetaxel via pregnane X receptor.; Authors - En-Qi Qiao
human	IHC	Reprod Sci; Protease Amplification of the Inflammatory Response Induced by Commensal Bacteria: Implications for Racial Disparity in Term and Preterm Birth.; Authors - Scott W Walsh