• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-NR1D1

NR1D1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugaison

Non conjugué

N° de cat : 14506-1-AP

Synonymes

ear 1, EAR1, hRev, NR1D1, Rev erbA alpha, Reverb alpha, THRA1, THRAL, V erbA related protein 1



Applications testées

Résultats positifs en WBcellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3, cellules Y79, tissu de muscle squelettique de souris
Résultats positifs en IPcellules A549
Résultats positifs en IF/ICCcellules NIH/3T3

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

14506-1-AP cible NR1D1 dans les applications de WB, IHC, IF/ICC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène NR1D1 Protéine recombinante Ag5964
Nom complet nuclear receptor subfamily 1, group D, member 1
Masse moléculaire calculée 67 kDa
Poids moléculaire observé 55-68 kDa
Numéro d’acquisition GenBankBC056148
Symbole du gène NR1D1
Identification du gène (NCBI) 9572
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.

Protocole

Product Specific Protocols
WB protocol for NR1D1 antibody 14506-1-APDownload protocol
IF protocol for NR1D1 antibody 14506-1-APDownload protocol
IP protocol for NR1D1 antibody 14506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Authors - Yi Liu
mouseWB

Theranostics

PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1.

Authors - Shuai Wang
humanWB,IF

Cancer Res

NR1D1 recruitment to sites of DNA damage inhibits repair and is associated with chemosensitivity of breast cancer.

Authors - Na-Lee Ka
mouseWB

Cell Death Discov

NR1D1 deficiency in the tumor microenvironment promotes lung tumor development by activating the NLRP3 inflammasome

Authors - Sun Mi Kim
mouseWB

J Neuroinflammation

The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome.

Authors - Liang Kou
humanWB

Cell Biosci

Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy.

Authors - Qingxue Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Priya (Verified Customer) (01-17-2023)

I have used for cardiomyocytes, mouse skin and liver tissues

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin
FH

Satish (Verified Customer) (12-19-2019)

I got clear bands. The antibody detects two band one band around 67 kDa and another band much lower than 67kDa.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: INS1 cells