- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-NR1D1
NR1D1 Polyclonal Antibody for WB, IP, IF, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (1)
Applications
WB, IHC, IF/ICC, IP, chIP, ELISA
Conjugaison
Non conjugué
N° de cat : 14506-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3, cellules Y79, tissu de muscle squelettique de souris |
Résultats positifs en IP | cellules A549 |
Résultats positifs en IF/ICC | cellules NIH/3T3 |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 3 publications below |
WB | See 21 publications below |
IHC | See 1 publications below |
IF | See 4 publications below |
IP | See 1 publications below |
ChIP | See 5 publications below |
Informations sur le produit
14506-1-AP cible NR1D1 dans les applications de WB, IHC, IF/ICC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | NR1D1 Protéine recombinante Ag5964 |
Nom complet | nuclear receptor subfamily 1, group D, member 1 |
Masse moléculaire calculée | 67 kDa |
Poids moléculaire observé | 55-68 kDa |
Numéro d’acquisition GenBank | BC056148 |
Symbole du gène | NR1D1 |
Identification du gène (NCBI) | 9572 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.
Protocole
Product Specific Protocols | |
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WB protocol for NR1D1 antibody 14506-1-AP | Download protocol |
IF protocol for NR1D1 antibody 14506-1-AP | Download protocol |
IP protocol for NR1D1 antibody 14506-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. | ||
Theranostics PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1. | ||
Cancer Res NR1D1 recruitment to sites of DNA damage inhibits repair and is associated with chemosensitivity of breast cancer. | ||
Cell Death Discov NR1D1 deficiency in the tumor microenvironment promotes lung tumor development by activating the NLRP3 inflammasome | ||
J Neuroinflammation The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome. | ||
Cell Biosci Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Priya (Verified Customer) (01-17-2023) | I have used for cardiomyocytes, mouse skin and liver tissues
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FH Satish (Verified Customer) (12-19-2019) | I got clear bands. The antibody detects two band one band around 67 kDa and another band much lower than 67kDa.
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