Anticorps Polyclonal de lapin anti-NPL

NPL Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 16715-1-AP

Synonymes

c112, C1orf13, N acetylneuraminate lyase, NALase, NPL, Sialate lyase, Sialate pyruvate lyase, Sialic acid aldolase, Sialic acid lyase

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Galerie de données de validation

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  WB analysis using 16715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 16715-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16715-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 16715-1-AP

  Immunofluorescent analysis of MCF-7 cells, using NPL antibody 16715-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Raji, tissu cérébral de rat, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16715-1-AP cible NPL dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPL Protéine recombinante Ag10137
• Nom complet: N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
• Masse moléculaire calculée: 27 kDa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC058003
• Symbole du gène: NPL
• Identification du gène (NCBI): 80896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.