Anticorps Polyclonal de lapin anti-NPHP3

NPHP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 22026-1-AP

Synonymes

KIAA2000, MKS7, Nephrocystin 3, NPHP3

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Galerie de données de validation

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  WB analysis using 22026-1-AP

  mouse testis lysates were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 22026-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22026-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 22026-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22026-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 22026-1-AP

  IP Result of anti-NPHP3 (IP:22026-1-AP, 4ug; Detection:22026-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human heart using 22026-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22026-1-AP (NPHP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 22026-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22026-1-AP (NPHP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 22026-1-AP

  IF result (the base and the lower half of cilia stain) of anti-NPHP3 (22026-1-AP, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

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  IF Staining of MDCK using 22026-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 22026-1-AP (NPHP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HEK-293, cellules HepG2, tissu hépatique de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules hTERT-RPE1, cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

22026-1-AP cible NPHP3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPHP3 Protéine recombinante Ag16888
• Nom complet: nephronophthisis 3 (adolescent)
• Masse moléculaire calculée: 1330 aa, 151 kDa
• Poids moléculaire observé: 145-151 kDa
• Numéro d’acquisition GenBank: BC068082
• Symbole du gène: NPHP3
• Identification du gène (NCBI): 27031
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Nat Methods; Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.; Authors - Su Steven S
human	IF	Am J Hum Genet; ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition.; Authors - Sumaya Alkanderi
human	IF	Hum Mol Genet; Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells.; Authors - Nele Schwarz
mouse	IF	Int J Mol Sci; Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses.; Authors - Shweta Suiwal
mouse	IF	Mol Biol Cell; Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia; Authors - Vivek Reddy Palicharla
		Methods Cell Biol; Single-molecule imaging in the primary cilium; Authors - Lucien E Weiss

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lelongt (Verified Customer) (12-10-2021)

Immunofluorescence is observed in mice cilia but is not restricted to the inversin compartment. Dilution has to be adjusted for WB since there are few bands.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1/100 immunofluoresecence mice. 1/1000 WB dog
• Cell Tissue Type: mouce parrafin sections (immunoflorescence). MDCK cells (Western blot)