Anticorps Polyclonal de lapin anti-NPC1

• Phare
• Validé par KD/KO

NPC1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

20

Avis (3)

N° de cat : 13926-1-AP

Synonymes

NPC intracellular cholesterol transporter 1, Niemann-Pick C1 protein, Niemann Pick disease, type C1, Niemann Pick C1 protein

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Galerie de données de validation

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  WB analysis using 13926-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13926-1-AP (NPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13926-1-AP

  WB result of NPC1 antibody (13926-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NPC1 transfected HEK-293 cells.

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  WB analysis using 13926-1-AP

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 13926-1-AP (NPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13926-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13926-1-AP (NPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13926-1-AP (NPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13926-1-AP (NPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13926-1-AP (NPC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13926-1-AP (NPC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13926-1-AP (NPC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13926-1-AP (NPC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of Neuro-2a using 13926-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using NPC1 antibody (13926-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 13926-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13926-1-AP (NPC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 13926-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NPC1 (13926-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293 non bouillies, cellules HEK-293, cellules HeLa, cellules HepG2, tissu cérébral de souris non bouilli
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Neuro-2a, cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IHC: See 2 publications below

Informations sur le produit

13926-1-AP cible NPC1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPC1 Protéine recombinante Ag4946
• Nom complet: Niemann-Pick disease, type C1
• Masse moléculaire calculée: 142 kDa
• Poids moléculaire observé: 160-200 kDa
• Numéro d’acquisition GenBank: BC063302
• Symbole du gène: NPC1
• Identification du gène (NCBI): 4864
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Niemann-Pick Type C (NPC) disease is a lysosomal storage disorder characterized by the accumulation of unesterified cholesterol and other lipids in the endolysosomal system. NPC disease results from a defect in either of two distinct cholesterol-binding proteins: a transmembrane protein, NPC1, and a small soluble protein, NPC2. NPC1 or NPC2 deficiency models showed that the functions of these two proteins within lysosomes are linked closely. NPC1 is a typical transmembrane protein and contains a number of modification sites for glycosylation. Defects in NPC1 are the cause of Niemann-Pick disease type C1 which exhibits highly variable clinical phenotype. Moreover, NPC1 may play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.

Publications

Species	Application	Title
human	WB	Cell; Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts.; Authors - Bei-Bei Chu ; KD Validated
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
human	WB	Proc Natl Acad Sci U S A; Cholesterol trafficking is required for mTOR activation in endothelial cells.; Authors - Xu Jing J ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.; Authors - Constance S Petit
mouse	WB	Cell Rep; The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.; Authors - Jian Wei

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parijat (Verified Customer) (04-08-2024)

Good antibody for specific staining of NPC1 in IF.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: MEF

FH

Ganesh (Verified Customer) (02-07-2024)

25 µg of HAP1 (human) whole cell lysate was resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. NPC1 antibody was diluted at 1:1000 in Blocking Buffer (5% Milk in 1x TBS-T (0.1% Tween-20)) and the membrane was incubated overnight at 4 deg C on a rotator.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HAP1

NPC1 Antibody Western Blot validation (1:1000 dilution) in HAP1 (Cat no:13926-1-AP)

FH

Benjamin (Verified Customer) (12-09-2021)

Nice antibody that detects Npc1 at the correct molecular weight in primary mouse neurons.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary Mouse Neurons

NPC1 Antibody Western Blot validation (1:1000 dilution) in Primary Mouse Neurons (Cat no:13926-1-AP)