Anticorps Polyclonal de lapin anti-NOX4

• Phare
• Validé par KD/KO

NOX4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

262

Avis (1)

N° de cat : 14347-1-AP

Synonymes

NOX4 Rabbit PolyAb, KOX 1, KOX, Kidney superoxide-producing NADPH oxidase, Kidney oxidase-1

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Galerie de données de validation

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  WB analysis using 14347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14347-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14347-1-AP (NOX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14347-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14347-1-AP (NOX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of BxPC-3 using 14347-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 14347-1-AP (NOX4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14347-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NOX4 (14347-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HEK-293, cellules HeLa, cellules JAR, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules BxPC-3
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 230 publications below
• IHC: See 43 publications below
• IF: See 24 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

14347-1-AP cible NOX4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOX4 Protéine recombinante Ag5687
• Nom complet: NADPH oxidase 4
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC040105
• Symbole du gène: NOX4
• Identification du gène (NCBI): 50507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. Studies have reported molecular masses of Nox4 protein by western blot analysis ranging from 55 to 80 kDa. The truncated NOX4 splice variant D (28 kDa) lacks the majority of the transmembrane domain and has been shown to produce higher levels of ROS and DNA damage compared to its prototype. NOX4D has previously been shown to localise to the nucleus and nucleolus in various cell types and is implicated in the generation of reactive oxygen species (ROS) and DNA damage. (PMID: 11728818, PMID: 29285262, PMID: 14670934)

Publications

Species	Application	Title
mouse	WB	Circulation; Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury.; Authors - Yu Liming
		Nat Commun; Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells.; Authors - Emre Balta
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu
human	WB,IF	Redox Biol; Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.; Authors - Hongtao Chen
rat	WB	Redox Biol; RND3 attenuates oxidative stress and vascular remodeling in spontaneously hypertensive rat via inhibiting ROCK1 signaling.; Authors - Nan Wu
human	WB	Autophagy; PMID: 28613983; Authors - Kazuya Tsubouchi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

K (Verified Customer) (02-02-2022)

This antibody gave us really nice bands in 1:1000 dilution with 5% BSA+TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela

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