- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-NOX1
NOX1 Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IHC, ELISA
Conjugaison
Non conjugué
N° de cat : 17772-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu rénal de souris, tissu rénal de rat |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below |
| WB | See 47 publications below |
| IHC | See 5 publications below |
Informations sur le produit
17772-1-AP cible NOX1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, porc, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | NOX1 Protéine recombinante Ag12187 |
| Nom complet | NADPH oxidase 1 |
| Masse moléculaire calculée | 564 aa, 65 kDa |
| Poids moléculaire observé | 59 kDa |
| Numéro d’acquisition GenBank | BC075014 |
| Symbole du gène | NOX1 |
| Identification du gène (NCBI) | 27035 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
NNOX1(NADPH oxidase 1) is also named as MOX1, NOH1 and belongs to the reactive oxygen species (ROS)-generating NADPH oxidase family. It plays a role in host defense, cell growth and differentiation, cell migration, and malignant transformation(PMID:20351171). It also can mediate oncogenic Ras-induced upregulation of VEGF and angiogenesis by activating Sp1 through Ras-ERK-dependent phosphorylation of Sp1(PMID:18454179). It has 3 isoforms produced by alternative splicing with the molecular weight of 65 kDa, 22 kDa and 59 kDa. The full length protein has two glycosylation sites. It always can be detected two bands of 63 kDa and 55 kDa in cells by western blot or another lower band of 45 kDa which is not likely due to alternative translation or splice variation, but as adjustment for the signal peptide and tag removal(PMID:17560373) or unspecifc(PMID:17460729).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for NOX1 antibody 17772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Crit Care Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein. | ||
Circulation Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury. | ||
Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice | ||
Redox Biol RND3 attenuates oxidative stress and vascular remodeling in spontaneously hypertensive rat via inhibiting ROCK1 signaling. | ||
Redox Biol p47phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis. | ||
Oxid Med Cell Longev Design, Synthesis, and Biological Evaluation of a Novel Aminothiol Compound as Potential Radioprotector. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Balawant (Verified Customer) (08-23-2022) | I have used this antibody for colon tissue sample and colon cell line like caco2 cells, HT29 cells , it is working excellent.
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