Anticorps Polyclonal de lapin anti-iNOS
iNOS Polyclonal Antibody for WB, FC (Intra), ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (2)
Applications
WB, IHC, FC (Intra), ELISA, IF
Conjugaison
Non conjugué
109
N° de cat : 22226-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules RAW 264.7, |
Résultats positifs en FC (Intra) | cellules RAW 264.7 traitées au LPS, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 85 publications below |
IHC | See 16 publications below |
IF | See 34 publications below |
Informations sur le produit
22226-1-AP cible iNOS dans les applications de WB, IHC, FC (Intra), ELISA, IF et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | rat, Humain, poulet, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | iNOS Protéine recombinante Ag17567 |
Nom complet | nitric oxide synthase 2, inducible |
Masse moléculaire calculée | 1153 aa, 131 kDa |
Poids moléculaire observé | 110-130 kDa, 65-70 kDa |
Numéro d’acquisition GenBank | BC130283 |
Symbole du gène | iNOS |
Identification du gène (NCBI) | 4843 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228).
Protocole
Product Specific Protocols | |
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WB protocol for iNOS antibody 22226-1-AP | Download protocol |
FC protocol for iNOS antibody 22226-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Ther Epsin1-mediated exosomal sorting of Dll4 modulates the tubular-macrophage crosstalk in diabetic nephropathy | ||
J Nanobiotechnology Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy | ||
Cancer Lett Inducible nitric oxide synthase (iNOS)-activated Cxcr2 signaling in myeloid cells promotes TGFβ-dependent squamous cell carcinoma lung metastasis | ||
J Neuroinflammation Disturbance of neuron-microglia crosstalk mediated by GRP78 in Neuropsychiatric systemic lupus erythematosus mice | ||
Cell Rep Roseburia intestinalis sensitizes colorectal cancer to radiotherapy through the butyrate/OR51E1/RALB axis | ||
Oxid Med Cell Longev Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2 |