Anticorps Polyclonal de lapin anti-NOP2

NOP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10448-1-AP

Synonymes

NOL1, NOP120, NOP2, NSUN1, Nucleolar protein 1, Nucleolar protein 2 homolog, p120

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Galerie de données de validation

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  WB analysis of A2780 using 10448-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10448-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10448-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10448-1-AP (NOP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10448-1-AP (NOP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10448-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 10448-1-AP (NOP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules C6, cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

10448-1-AP cible NOP2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOP2 Protéine recombinante Ag0498
• Nom complet: NOP2 nucleolar protein homolog (yeast)
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC000656
• Symbole du gène: NOP2
• Identification du gène (NCBI): 4839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOL1, (synonyms: p120, NSUN1, NOP120), is a 120 kDa proliferating-cell nucleolar antigen and is the most cancer specific of the proliferation-associated nucleolar proteins identified thus far. NOL1 is expressed in G1 and peaks during the early S phase of the cell cycle and it has not been detected in benign tumors and most normal resting tissues. Overexpression of NOL1 caused the transformation of NIH 3T3 cells and expression of an antisense NOL1 construct inhibited the growth of NIH 3T3 cells. NOL is localized in a novel nucleolar microfibrillar structure, and contains, consecutively, four major domains: a basic domain, an acidic domain, a hydrophobic and methionine-rich domain, and a domain rich in cysteine and proline residues. The gene for human NOL1 was assigned to chromosome 12p13.

Publications

Species	Application	Title
human	WB,IF	Nucleic Acids Res; DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1.; Authors - Calkins Anne S AS
human	WB	Mol Biol Cell; RRP1B targets PP1 to mammalian cell nucleoli and is associated with Pre-60S ribosomal subunits.; Authors - Chamousset Delphine D
human	WB	Transl Cancer Res; Effect of NOP2 knockdown on colon cancer cell proliferation, migration, and invasion.; Authors - Jinling Bi
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi
mouse	IF	Cell Mol Life Sci; N4-acetylcytidine of Nop2 mRNA is required for the transition of morula-to-blastocyst; Authors - Mengyun Wang
human	WB,IF,IP	Cell Rep; ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells; Authors - M Carmen Lafita-Navarro