Anticorps Polyclonal de lapin anti-NOMO1

NOMO1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 17792-1-AP

Synonymes

NODAL modulator 1, Nomo, NOMO1, PM5, pM5 protein

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Galerie de données de validation

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  WB analysis of mouse colon using 17792-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17792-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17792-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17792-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17792-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17792-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 17792-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17792-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17792-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 17792-1-AP

  IP result of anti-NOMO1 (IP:17792-1-AP, 4ug; Detection:17792-1-AP 1:1000) with A431 cells lysate 1520ug.

• 

  IHC staining of human colon using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

• 

  IF Staining of MCF-7 using 17792-1-AP

  Immunofluorescent analysis of MCF-7 cells, using NOMO1 antibody 17792-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, cellules A431, cellules COLO 320, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du côlon humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

17792-1-AP cible NOMO1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOMO1 Protéine recombinante Ag11895
• Nom complet: NODAL modulator 1
• Masse moléculaire calculée: 1222 aa, 134 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC065535
• Symbole du gène: NOMO1
• Identification du gène (NCBI): 23420
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Clin Sci (Lond); DLX3 promotes bone marrow mesenchymal stem cell proliferation through H19/miR-675 axis.; Authors - Na Zhao