Anticorps Polyclonal de lapin anti-NOB1

NOB1 Polyclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14456-1-AP

Synonymes

NOB1P, MSTP158, MST158, EC:3.1.-.-, ART4

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Galerie de données de validation

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  WB analysis of Jurkat using 14456-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14456-1-AP (NOB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14456-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14456-1-AP (NOB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 14456-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug NOB1 Polyclonal antibody (14456-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules L02
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14456-1-AP cible NOB1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOB1 Protéine recombinante Ag6051
• Nom complet: NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC064630
• Symbole du gène: NOB1
• Identification du gène (NCBI): 28987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.

Publications

Species	Application	Title
human,mouse	WB	Am J Transl Res; miRNA-612 suppresses ovarian cancer cell tumorigenicity by downregulating NOB1.; Authors - Zhikun Shi