Anticorps Polyclonal de lapin anti-NMNAT3

NMNAT3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 13236-1-AP

Synonymes

NaMN adenylyltransferase 3, NMNAT3, PNAT 3, PNAT3

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Galerie de données de validation

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  WB analysis of mouse kidney using 13236-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13236-1-AP (NMNAT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13236-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13236-1-AP (NMNAT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

13236-1-AP cible NMNAT3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NMNAT3 Protéine recombinante Ag3965
• Nom complet: nicotinamide nucleotide adenylyltransferase 3
• Masse moléculaire calculée: 215 aa, 24 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC034374
• Symbole du gène: NMNAT3
• Identification du gène (NCBI): 349565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Int J Biol Sci; Nicotinamide Riboside Enhances Mitochondrial Proteostasis and Adult Neurogenesis through Activation of Mitochondrial Unfolded Protein Response Signaling in the Brain of ALS SOD1G93A Mice.; Authors - Qi Zhou
mouse	WB	Food Funct; β-Nicotinamide mononucleotide supplementation prolongs the lifespan of prematurely aged mice and protects colon function in ageing mice; Authors - Yanrou Gu
mouse,rat	WB	EMBO J; Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function; Authors - Quanjiang Zhang
mouse	IHC	Int J Mol Sci; Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1; Authors - Kazuhiro Hasegawa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Caitlin (Verified Customer) (01-09-2020)

I used the NMNAT3 antibody for protein detection via Western Blot on mouse DRG neuronal lysate. The antibody was able to detect my protein of interest, but the bands were very faint (likely due to low protein concentration on my end).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: mouse neurons