Anticorps Polyclonal de lapin anti-NMD3

NMD3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16060-1-AP

Synonymes

CGI 07, hNMD3, NMD3

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Galerie de données de validation

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  WB analysis using 16060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16060-1-AP (NMD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16060-1-AP

  IP result of anti-NMD3 (IP:16060-1-AP, 3ug; Detection:16060-1-AP 1:800) with HepG2 cells lysate 1720ug.

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  IHC staining of human breast cancer using 16060-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16060-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16060-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16060-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 16060-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16060-1-AP (NMD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

16060-1-AP cible NMD3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NMD3 Protéine recombinante Ag8894
• Nom complet: NMD3 homolog (S. cerevisiae)
• Masse moléculaire calculée: 503 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC013317
• Symbole du gène: NMD3
• Identification du gène (NCBI): 51068
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human NMD3 gene encodes 60S ribosomal export protein which was found in a 60S ribosomal subunit export complex with RAN and XPO1. This nucleocytoplasmic shuttling protein NMD3 is an adaptor for export of the 60S ribosomal subunit from the nucleus. NMD3 contains a CRM-1-dependent leucine-rich nuclear export signal (NES) and a dispersed nuclear localization signal (NLS), the basic region of which is also required for nucleolar accumulation. NMD3 are required for nuclear export of the 60S ribosomal subunit in yeast and vertebrate cells, recent finding has also revealed its role in Arabidopsis thaliana.

Publications

Species	Application	Title
human	WB	Cell Stem Cell; HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.; Authors - Kaosheng Lv
mouse	WB	Genes Dev; Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.; Authors - Andrew J Finch
human	WB	Blood; Defective ribosome assembly in Shwachman-Diamond syndrome.; Authors - Wong Chi C CC
human	IF	Nucleic Acids Res; The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis; Authors - Matthew John Eastham
human	WB	Cancer Lett; RNA-binding protein hnRNPU regulates multiple myeloma resistance to selinexor; Authors - Xin Wang
human,mouse	WB	Nature; Synthesis of portimines reveals the basis of their anti-cancer activity; Authors - Junchen Tang ; KD Validated