Anticorps Polyclonal de lapin anti-neuromedin B

neuromedin B Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10888-1-AP

Synonymes

neuromedin B, NMB

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Galerie de données de validation

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  WB analysis of Transfected HEK-293 using 10888-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10888-1-AP (neuromedin B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10888-1-AP (neuromedin B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:25 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu cutané humain, tissu de cancer du sein humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below

Informations sur le produit

10888-1-AP cible neuromedin B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, singe, Chimpanzé
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: neuromedin B Protéine recombinante Ag0842
• Nom complet: neuromedin B
• Masse moléculaire calculée: 16 kDa
• Numéro d’acquisition GenBank: BC007407
• Symbole du gène: NMB
• Identification du gène (NCBI): 4828
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neuromedin B (NMB) is a bombesin-like peptide, which inhibits food intake and modulates stress-related behaviour. It exerts its function via NMB receptor (NMB-R). NMB is encoded in a 76-amino acid precursor. The mature NMB was reported to be a 10-amino-acid peptide, giving two bands of 1.2 kDa and 1.5 kDa in western blotting. NMB is expressed in brain, gastrointestinal tract, pancreas, adrenals, and adipose tissue. It regulates exocrine and endocrine secretions, cell growth, body temperature and blood pressure and glucose level in vivo.

Publications

Species	Application	Title
chimpanzee	IHC	Brain Struct Funct; The von Economo neurons in frontoinsular and anterior cingulate cortex in great apes and humans.; Authors - Allman John M JM
human	IHC	Cancer Lett; Neuromedin B receptor antagonist suppresses tumor angiogenesis and tumor growth in vitro and in vivo.; Authors - Park Hyun-Joo HJ
human,monkey	WB	Am J Hum Biol; Biochemical specificity of von Economo neurons in hominoids.; Authors - Stimpson Cheryl D CD