Anticorps Monoclonal anti-TTF1

TTF1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, porc

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2C8F3

N° de cat : 66034-1-Ig

Synonymes

BHC, Homeobox protein Nkx 2.1, NK 2, NK2 homeobox 1, NKX2 1, NKX2.1, NKX2-1, NKX2A, TEBP, Thyroid nuclear factor 1, Thyroid transcription factor 1, TITF1, TTF 1, TTF1

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Galerie de données de validation

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  WB analysis of HepG2 using 66034-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66034-1-Ig (TTF1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 66034-1-Ig(NKX2-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 66034-1-Ig(NKX2-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 66034-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using TTF1 antibody (66034-1-Ig, Clone: 2C8F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du poumon humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

66034-1-Ig cible TTF1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: NK2 homeobox 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: NM_003317
• Symbole du gène: NKX2-1
• Identification du gène (NCBI): 7080
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Homeobox protein Nkx-2.1 (NKX2-1), also named Thyroid nuclear factor 1,Thyroid transcription factor 1(TTF1). It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD).

Publications

Species	Application	Title
human	WB	ACS Omega; Interpreting the Pharmacological Mechanisms of Sho-saiko-to on Thyroid Carcinoma through Combining Network Pharmacology and Experimental Evaluation.; Authors - Kun Wang
human	WB	Cell Biochem Biophys; Suberoylanilide Hydroxamic Acid (SAHA) Reduces Fibrosis Markers and Deactivates Human Stellate Cells via the Epithelial-Mesenchymal Transition (EMT).; Authors - Merve Özel
human	IHC	Nat Commun; Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features; Authors - Weizhu Zhao