Anticorps Polyclonal de lapin anti-NKIRAS2

NKIRAS2 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 10174-1-AP

Synonymes

Kappa B Ras protein 2, kappaB Ras2, KBRAS2, NKIRAS2

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 10174-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10174-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of HEK-293 using 10174-1-AP

  IP result of anti-NKIRAS2 (IP:10174-1-AP, 4ug; Detection:10174-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

• 

  IF Staining of A431 using 10174-1-AP

  Immunofluorescent analysis of A431 cells using 10174-1-AP (NKIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

10174-1-AP cible NKIRAS2 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NKIRAS2 Protéine recombinante Ag0221
• Nom complet: NFKB inhibitor interacting Ras-like 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC007450
• Symbole du gène: NKIRAS2
• Identification du gène (NCBI): 28511
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

I-Kappa-B-interacting Ras-like protein 2 (KBRAS2, MGC:4553), interact with the PEST domains of IkappaBalpha and IkappaBbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappaB)] and decrease their rate of degradation. KBRAS2 differs from other Ras proteins in containing Ala and Leu residues at positions 12 and 61 respectively, which are similar to those present in the oncogenic forms of Ras.

Publications

Species	Application	Title
human	WB	Nat Commun; κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis.; Authors - Stephanie Beel

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Andrea (Verified Customer) (09-18-2020)

The antibody works great in Western Blot. Good signal and specificity, which was tested on knockout cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreas, different human and mouse cell lines