Anticorps Polyclonal de lapin anti-NIPBL

NIPBL Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 18792-1-AP

Synonymes

CDLS, CDLS1, Delangin, IDN3, IDN3 B, NIPBL, Nipped B homolog (Drosophila), Nipped B like protein, SCC2 homolog

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Galerie de données de validation

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  WB analysis of HeLa using 18792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 18792-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18792-1-AP (NIPBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

18792-1-AP cible NIPBL dans les applications de WB, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NIPBL Protéine recombinante Ag5663
• Nom complet: Nipped-B homolog (Drosophila)
• Masse moléculaire calculée: 2804 aa, 316 kDa
• Poids moléculaire observé: 300 kDa
• Numéro d’acquisition GenBank: BC033847
• Symbole du gène: NIPBL
• Identification du gène (NCBI): 25836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.

Publications

Species	Application	Title
mouse	ChIP	Cell; Lineage-specific 3D genome organization is assembled at multiple scales by IKAROS; Authors - Yeguang Hu
human	WB	Nat Commun; C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.; Authors - Dan Liu
mouse	ChIP	Nat Immunol; BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin; Authors - Malay Mandal
mouse	WB,IP	Nucleic Acids Res; BRD2 promotes antibody class switch recombination by facilitating DNA repair in collaboration with NIPBL; Authors - Santosh K Gothwal