Anticorps Polyclonal de lapin anti-NHP2L1

• Phare
• Validé par KD/KO

NHP2L1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15802-1-AP

Synonymes

15.5K, FA 1, FA1, hSNU13, NHP2 like protein 1, NHP2L1, NHPX, OTK27, SNRNP15 5, SNU13, SNU13 homolog, SPAG12, SSFA1

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Galerie de données de validation

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  WB analysis using 15802-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15802-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15802-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 15802-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A431 using 15802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NHP2L1 antibody (15802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

15802-1-AP cible NHP2L1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NHP2L1 Protéine recombinante Ag8532
• Nom complet: NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)
• Masse moléculaire calculée: 128 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC005358
• Symbole du gène: NHP2L1
• Identification du gène (NCBI): 4809
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NHP2L1, belongs to the ribosomal protein L7Ae family, is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs, and is required for subsequent recruitment of PRP31 and activation of the spliceosomal complex [PMID: 17412961]. It binds to the 5'-stem-loop of U4 snRNA and may have a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding [PMID: 10545122].

Publications

Species	Application	Title
human	WB	Mol Cell; Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.; Authors - Run-Wen Yao ; KO Validated
human	IF	Mol Cell; Condensates induced by transcription inhibition localize active chromatin to nucleoli.; Authors - Takaaki Yasuhara
human	WB	Mol Cell; LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.; Authors - Xin Wang
mouse	WB	Biochim Biophys Acta Proteins Proteom; Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound.; Authors - Uchenna Emechebe