Anticorps Polyclonal de lapin anti-NGDN

NGDN Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 16524-1-AP

Synonymes

C14orf120, EIF4E binding protein, LCP5, lpd 2, Neuroguidin, NGD, NGDN

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Galerie de données de validation

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  WB analysis of human cerebellum using 16524-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16524-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human epididymis using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human epididymis using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16524-1-AP

  Immunofluorescent analysis of Hela cells, using NGDN antibody 16524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu de cervelet humain, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu d'épididyme humain, tissu cardiaque humain, tissu hépatique humain, tissu placentaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16524-1-AP cible NGDN dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NGDN Protéine recombinante Ag9763
• Nom complet: neuroguidin, EIF4E binding protein
• Masse moléculaire calculée: 315 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC030817
• Symbole du gène: NGDN
• Identification du gène (NCBI): 25983
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Nature; A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis.; Authors - Carlos Gorbea