Anticorps Polyclonal de lapin anti-NFS1

• Phare
• Validé par KD/KO

NFS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15370-1-AP

Synonymes

HUSSY 08, IscS, NFS1, NIFS

---

Galerie de données de validation

• 

  WB analysis of A549 using 15370-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15370-1-AP (NFS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15370-1-AP

  WB result of NFS1 antibody (15370-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFS1 transfected HepG2 cells.

• 

  WB analysis of HepG2 using 15370-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15370-1-AP (NFS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15370-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15370-1-AP (NFS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15370-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15370-1-AP (NFS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15370-1-AP

  IP result of anti-NFS1 (IP:15370-1-AP, 4ug; Detection:15370-1-AP 1:500) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human liver using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15370-1-AP

  Immunofluorescent analysis of HepG2 cells, using NFS1 antibody 15370-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 15370-1-AP

  Immunofluorescent analysis of HepG2 cells using 15370-1-AP (NFS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, tissu cardiaque humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15370-1-AP cible NFS1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFS1 Protéine recombinante Ag3872
• Nom complet: NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC018471
• Symbole du gène: NFS1
• Identification du gène (NCBI): 9054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.

Publications

Species	Application	Title
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
mouse	WB	J Nanobiotechnology; Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.; Authors - Li Xu
mouse	WB	FASEB J; PMID: 31530015; Authors - Rufeng Zhang
human	WB,IF	PLoS One; Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.; Authors - Ioana Ferecatu ; KD Validated
human	IHC	Discov Oncol; L-cysteine contributes to destructive activities of odontogenic cysts/tumor; Authors - Ji Li
human	WB	Cancers (Basel); Ferroptosis Inducers Kill Mesenchymal Stem Cells Affected by Neuroblastoma; Authors - Xiangze Li