Anticorps Polyclonal de lapin anti-NFKBIZ

• Phare
• Validé par KD/KO

NFKBIZ Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 14014-1-AP

Synonymes

I kappa B zeta, IkappaB zeta, IkappaBzeta, IkB zeta, IKBZ, INAP, IκBζ, MAIL, NF kappa B inhibitor zeta, NFKBIZ

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Galerie de données de validation

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  WB analysis of HeLa using 14014-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14014-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14014-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14014-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IF Staining of HeLa using 14014-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFKBIZ antibody (14014-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules THP-1, cellules U-251
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14014-1-AP cible NFKBIZ dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFKBIZ Protéine recombinante Ag5120
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 78 kDa, 64 kDa
• Numéro d’acquisition GenBank: BC060800
• Symbole du gène: NFKBIZ
• Identification du gène (NCBI): 64332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFKBIZ, also named as NF-kappa-B inhibitor zeta, is a 718 amino acid protein, which contains 7 ANK repeats and is expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. NFKBIZ exists as three isoforms and molecular weight of isoform three is a 64 kDa. The isoform one is 78 kDa protein and isoform two is a 68 kDa protein. NFKBIZ is involved in regulation of NF-kappa-B transcription factor complexes. It Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation.

Publications

Species	Application	Title
human	WB	Front Immunol; Single-Cell Transcriptome Analysis of Chronic Antibody-Mediated Rejection After Renal Transplantation.; Authors - Fanhua Kong
human	WB	Front Mol Neurosci; Silencing NUDT21 Attenuates the Mesenchymal Identity of Glioblastoma Cells via the NF-κB Pathway.; Authors - Jia-Cheng Lou
mouse	WB	Toxicol Appl Pharmacol; Tetrachloro-p-benzoquinone induces hepatic oxidative damage and inflammatory response, but not apoptosis in mouse: The prevention of curcumin.; Authors - Demei Xu
		Int J Biol Macromol; Charaterization and immunomodulatory activities of polysaccharide isolated from Pleurotus eryngii.; Authors - Duoduo Xu
rat	WB	J Surg Res; PMID: 25103639; Authors - Yu Zhang
mouse	IF	JCI Insight; The negative feedback loop of NF-κB/miR-376b/NFKBIZ in septic acute kidney injury.; Authors - Zhiwen Liu ; KD Validated