Anticorps Polyclonal de lapin anti-IkB Alpha

IkB Alpha Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

470

Avis (2)

N° de cat : 10268-1-AP

Synonymes

IKBA, IκB α, NFKBIA, Ikβ-α, IkB-alpha

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Galerie de données de validation

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  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10268-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 10268-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10268-1-AP

  IP result of anti-IkB Alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:15000) with HeLa cells lysate 1320 ug.

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 10268-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10268-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10268-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IkB Alpha (10268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HL-60, cellules Jurkat, cellules NIH/3T3, cellules PC-12, tissu hépatique de porc
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer de la prostate humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 449 publications below
• IHC: See 14 publications below
• IF: See 7 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

10268-1-AP cible IkB Alpha dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IkB Alpha Protéine recombinante Ag0345
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC002601
• Symbole du gène: IkB Alpha
• Identification du gène (NCBI): 4792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human	WB	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB,IF	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
mouse	WB	Sci Adv; Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice.; Authors - Yuanjiu Lei
human	WB	Genes Dev; PMID: 30463905; Authors - Rosa M Rubio

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SONAM (Verified Customer) (10-04-2023)

Antibody worked well. No nonspecific band was observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cells

FH

CHAO (Verified Customer) (01-30-2020)

Weak bands for the heart lysates at basal state.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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